RustEffectorV1, Main, Exploration, bibRecord, 000068

Effectors from Wheat Rust Fungi Suppress Multiple Plant Defense Responses.

Identifieur interne : 000068 ( Main/Exploration ); précédent : 000067; suivant : 000069

Effectors from Wheat Rust Fungi Suppress Multiple Plant Defense Responses.

Auteurs : Sowmya R. Ramachandran [États-Unis] ; Chuntao Yin [États-Unis] ; Joanna Kud [États-Unis] ; Kiwamu Tanaka [États-Unis] ; Aaron K. Mahoney [États-Unis] ; Fangming Xiao [États-Unis] ; Scot H. Hulbert [États-Unis]

Source :

Phytopathology [ 0031-949X ] ; 2017.

RBID : pubmed:27503371

Descripteurs français

English descriptors

KwdEn :
- Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Basidiomycota (genetics), Basidiomycota (physiology), Cell Death (MeSH), Gene Expression (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Suppressor (MeSH), Host-Pathogen Interactions (MeSH), Hypersensitivity (MeSH), Plant Diseases (immunology), Plant Diseases (microbiology), Plant Immunity (MeSH), Plant Leaves (genetics), Plant Leaves (immunology), Plant Leaves (microbiology), Plants, Genetically Modified (MeSH), Pseudomonas fluorescens (genetics), Pseudomonas fluorescens (physiology), Reactive Oxygen Species (metabolism), Tobacco (genetics), Tobacco (immunology), Tobacco (microbiology), Transcriptome (MeSH), Triticum (genetics), Triticum (immunology), Triticum (microbiology).
MESH :
- chemical , genetics : Bacterial Proteins.
- chemical , metabolism : Bacterial Proteins, Reactive Oxygen Species.
- genetics : Basidiomycota, Plant Leaves, Pseudomonas fluorescens, Tobacco, Triticum.
- immunology : Plant Diseases, Plant Leaves, Tobacco, Triticum.
- microbiology : Plant Diseases, Plant Leaves, Tobacco, Triticum.
- physiology : Basidiomycota, Pseudomonas fluorescens.
- Cell Death, Gene Expression, Gene Expression Regulation, Plant, Genes, Suppressor, Host-Pathogen Interactions, Hypersensitivity, Plant Immunity, Plants, Genetically Modified, Transcriptome.

Abstract

Fungi that cause cereal rust diseases (genus Puccinia) are important pathogens of wheat globally. Upon infection, the fungus secretes a number of effector proteins. Although a large repository of putative effectors has been predicted using bioinformatic pipelines, the lack of available high-throughput effector screening systems has limited functional studies on these proteins. In this study, we mined the available transcriptomes of Puccinia graminis and P. striiformis to look for potential effectors that suppress host hypersensitive response (HR). Twenty small (<300 amino acids), secreted proteins, with no predicted functions were selected for the HR suppression assay using Nicotiana benthamiana, in which each of the proteins were transiently expressed and evaluated for their ability to suppress HR caused by four cytotoxic effector-R gene combinations (Cp/Rx, ATR13/RPP13, Rpt2/RPS-2, and GPA/RBP-1) and one mutated R gene-Pto(Y207D). Nine out of twenty proteins, designated Shr1 to Shr9 (suppressors of hypersensitive response), were found to suppress HR in N. benthamiana. These effectors varied in the effector-R gene defenses they suppressed, indicating these pathogens can interfere with a variety of host defense pathways. In addition to HR suppression, effector Shr7 also suppressed PAMP-triggered immune response triggered by flg22. Finally, delivery of Shr7 through Pseudomonas fluorescens EtHAn suppressed nonspecific HR induced by Pseudomonas syringae DC3000 in wheat, confirming its activity in a homologous system. Overall, this study provides the first evidence for the presence of effectors in Puccinia species suppressing multiple plant defense responses.

DOI: 10.1094/PHYTO-02-16-0083-R
PubMed: 27503371

Affiliations:

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Le document en format XML

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<term>Basidiomycota (physiology)</term>
<term>Cell Death (MeSH)</term>
<term>Gene Expression (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, Suppressor (MeSH)</term>
<term>Host-Pathogen Interactions (MeSH)</term>
<term>Hypersensitivity (MeSH)</term>
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<term>Plant Leaves (microbiology)</term>
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<term>Pseudomonas fluorescens (physiology)</term>
<term>Reactive Oxygen Species (metabolism)</term>
<term>Tobacco (genetics)</term>
<term>Tobacco (immunology)</term>
<term>Tobacco (microbiology)</term>
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<term>Feuilles de plante (immunologie)</term>
<term>Feuilles de plante (microbiologie)</term>
<term>Gènes suppresseurs (MeSH)</term>
<term>Hypersensibilité (MeSH)</term>
<term>Immunité des plantes (MeSH)</term>
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<term>Protéines bactériennes (métabolisme)</term>
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<term>Pseudomonas fluorescens (physiologie)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
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<term>Tabac (immunologie)</term>
<term>Tabac (microbiologie)</term>
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<term>Triticum (immunologie)</term>
<term>Triticum (microbiologie)</term>
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<front><div type="abstract" xml:lang="en">Fungi that cause cereal rust diseases (genus Puccinia) are important pathogens of wheat globally. Upon infection, the fungus secretes a number of effector proteins. Although a large repository of putative effectors has been predicted using bioinformatic pipelines, the lack of available high-throughput effector screening systems has limited functional studies on these proteins. In this study, we mined the available transcriptomes of Puccinia graminis and P. striiformis to look for potential effectors that suppress host hypersensitive response (HR). Twenty small (<300 amino acids), secreted proteins, with no predicted functions were selected for the HR suppression assay using Nicotiana benthamiana, in which each of the proteins were transiently expressed and evaluated for their ability to suppress HR caused by four cytotoxic effector-R gene combinations (Cp/Rx, ATR13/RPP13, Rpt2/RPS-2, and GPA/RBP-1) and one mutated R gene-Pto(Y207D). Nine out of twenty proteins, designated Shr1 to Shr9 (suppressors of hypersensitive response), were found to suppress HR in N. benthamiana. These effectors varied in the effector-R gene defenses they suppressed, indicating these pathogens can interfere with a variety of host defense pathways. In addition to HR suppression, effector Shr7 also suppressed PAMP-triggered immune response triggered by flg22. Finally, delivery of Shr7 through Pseudomonas fluorescens EtHAn suppressed nonspecific HR induced by Pseudomonas syringae DC3000 in wheat, confirming its activity in a homologous system. Overall, this study provides the first evidence for the presence of effectors in Puccinia species suppressing multiple plant defense responses.</div>
</front>
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<DateCompleted><Year>2017</Year>
<Month>06</Month>
<Day>29</Day>
</DateCompleted>
<DateRevised><Year>2017</Year>
<Month>06</Month>
<Day>29</Day>
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<JournalIssue CitedMedium="Print"><Volume>107</Volume>
<Issue>1</Issue>
<PubDate><Year>2017</Year>
<Month>Jan</Month>
</PubDate>
</JournalIssue>
<Title>Phytopathology</Title>
<ISOAbbreviation>Phytopathology</ISOAbbreviation>
</Journal>
<ArticleTitle>Effectors from Wheat Rust Fungi Suppress Multiple Plant Defense Responses.</ArticleTitle>
<Pagination><MedlinePgn>75-83</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Fungi that cause cereal rust diseases (genus Puccinia) are important pathogens of wheat globally. Upon infection, the fungus secretes a number of effector proteins. Although a large repository of putative effectors has been predicted using bioinformatic pipelines, the lack of available high-throughput effector screening systems has limited functional studies on these proteins. In this study, we mined the available transcriptomes of Puccinia graminis and P. striiformis to look for potential effectors that suppress host hypersensitive response (HR). Twenty small (<300 amino acids), secreted proteins, with no predicted functions were selected for the HR suppression assay using Nicotiana benthamiana, in which each of the proteins were transiently expressed and evaluated for their ability to suppress HR caused by four cytotoxic effector-R gene combinations (Cp/Rx, ATR13/RPP13, Rpt2/RPS-2, and GPA/RBP-1) and one mutated R gene-Pto(Y207D). Nine out of twenty proteins, designated Shr1 to Shr9 (suppressors of hypersensitive response), were found to suppress HR in N. benthamiana. These effectors varied in the effector-R gene defenses they suppressed, indicating these pathogens can interfere with a variety of host defense pathways. In addition to HR suppression, effector Shr7 also suppressed PAMP-triggered immune response triggered by flg22. Finally, delivery of Shr7 through Pseudomonas fluorescens EtHAn suppressed nonspecific HR induced by Pseudomonas syringae DC3000 in wheat, confirming its activity in a homologous system. Overall, this study provides the first evidence for the presence of effectors in Puccinia species suppressing multiple plant defense responses.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ramachandran</LastName>
<ForeName>Sowmya R</ForeName>
<Initials>SR</Initials>
<AffiliationInfo><Affiliation>First, second, fourth, fifth, and seventh authors: Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430; and third and sixth authors: Plant, Soil and Entomological Sciences, University of Idaho, Moscow, ID 83844-2339.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yin</LastName>
<ForeName>Chuntao</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>First, second, fourth, fifth, and seventh authors: Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430; and third and sixth authors: Plant, Soil and Entomological Sciences, University of Idaho, Moscow, ID 83844-2339.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kud</LastName>
<ForeName>Joanna</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>First, second, fourth, fifth, and seventh authors: Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430; and third and sixth authors: Plant, Soil and Entomological Sciences, University of Idaho, Moscow, ID 83844-2339.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tanaka</LastName>
<ForeName>Kiwamu</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>First, second, fourth, fifth, and seventh authors: Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430; and third and sixth authors: Plant, Soil and Entomological Sciences, University of Idaho, Moscow, ID 83844-2339.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mahoney</LastName>
<ForeName>Aaron K</ForeName>
<Initials>AK</Initials>
<AffiliationInfo><Affiliation>First, second, fourth, fifth, and seventh authors: Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430; and third and sixth authors: Plant, Soil and Entomological Sciences, University of Idaho, Moscow, ID 83844-2339.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xiao</LastName>
<ForeName>Fangming</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>First, second, fourth, fifth, and seventh authors: Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430; and third and sixth authors: Plant, Soil and Entomological Sciences, University of Idaho, Moscow, ID 83844-2339.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hulbert</LastName>
<ForeName>Scot H</ForeName>
<Initials>SH</Initials>
<AffiliationInfo><Affiliation>First, second, fourth, fifth, and seventh authors: Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430; and third and sixth authors: Plant, Soil and Entomological Sciences, University of Idaho, Moscow, ID 83844-2339.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>10</Month>
<Day>18</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Phytopathology</MedlineTA>
<NlmUniqueID>9427222</NlmUniqueID>
<ISSNLinking>0031-949X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016923" MajorTopicYN="N">Cell Death</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016153" MajorTopicYN="N">Genes, Suppressor</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054884" MajorTopicYN="Y">Host-Pathogen Interactions</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006967" MajorTopicYN="N">Hypersensitivity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057865" MajorTopicYN="Y">Plant Immunity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011551" MajorTopicYN="N">Pseudomonas fluorescens</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">reactive oxygen species</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2016</Year>
<Month>10</Month>
<Day>19</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>7</Month>
<Day>1</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2016</Year>
<Month>8</Month>
<Day>10</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">27503371</ArticleId>
<ArticleId IdType="doi">10.1094/PHYTO-02-16-0083-R</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Idaho</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Idaho"><name sortKey="Ramachandran, Sowmya R" sort="Ramachandran, Sowmya R" uniqKey="Ramachandran S" first="Sowmya R" last="Ramachandran">Sowmya R. Ramachandran</name>
</region>
<name sortKey="Hulbert, Scot H" sort="Hulbert, Scot H" uniqKey="Hulbert S" first="Scot H" last="Hulbert">Scot H. Hulbert</name>
<name sortKey="Kud, Joanna" sort="Kud, Joanna" uniqKey="Kud J" first="Joanna" last="Kud">Joanna Kud</name>
<name sortKey="Mahoney, Aaron K" sort="Mahoney, Aaron K" uniqKey="Mahoney A" first="Aaron K" last="Mahoney">Aaron K. Mahoney</name>
<name sortKey="Tanaka, Kiwamu" sort="Tanaka, Kiwamu" uniqKey="Tanaka K" first="Kiwamu" last="Tanaka">Kiwamu Tanaka</name>
<name sortKey="Xiao, Fangming" sort="Xiao, Fangming" uniqKey="Xiao F" first="Fangming" last="Xiao">Fangming Xiao</name>
<name sortKey="Yin, Chuntao" sort="Yin, Chuntao" uniqKey="Yin C" first="Chuntao" last="Yin">Chuntao Yin</name>
</country>
</tree>
</affiliations>
</record>

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   |texte=   Effectors from Wheat Rust Fungi Suppress Multiple Plant Defense Responses.
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	Serveur d'exploration sur les effecteurs de la rouille
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les effecteurs de la rouille

Effectors from Wheat Rust Fungi Suppress Multiple Plant Defense Responses.

Effectors from Wheat Rust Fungi Suppress Multiple Plant Defense Responses.

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